Application: ELISA
Reactivity:Rabbit
Sensitivity:5 pg/ml
Storage:2-8℃,6 months
Array ( [61] => Array ( [id] => 61 [name] => ELISA [children] => Array ( [117] => Array ( [id] => 117 [name] => Human ) [158] => Array ( [id] => 158 [name] => Mouse ) [157] => Array ( [id] => 157 [name] => Rats ) [776] => Array ( [id] => 776 [name] => Common Species ) [159] => Array ( [id] => 159 [name] => Other Species ) [777] => Array ( [id] => 777 [name] => Unpackaged Kit ) [238] => Array ( [id] => 238 [name] => Antibody Pairs ) [754] => Array ( [id] => 754 [name] => Related Auxiliary Reagent ) ) [num] => 0 ) )
Application: ELISA
Reactivity:Rabbit
Sensitivity:5 pg/ml
Storage:2-8℃,6 months
Chinese Name | 兔基质金属蛋白酶9检测试剂盒 |
English Name | Rabbit MMP-9 ELISA Kit |
Synonyms | 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase |
Storage | 2-8℃,6 months |
Detection Target | MMP-9 |
Sample Type | Serum,Plasma,Cell Culture Supernatant |
Reactivity | Rabbit |
Application | ELISA |
Reaction Type | Sandwich-ELISA |
Quantitative/Qualitative | Quantitative |
Detection Range | 12.5-800 pg/ml |
Sensitivity | 5 pg/ml |
Specificity | Do not react with ApoAI,BMP1,BMP2,BMP3,BMP4,BMP7,CRP,HGF,HSP27,IL-1α,IL-1RI,IL-1β,IL-1RA,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12,IL-13,IL-15,IL-17C,IL-21,IL-23,MMP-2,sIL-2R,sIL-6R,PDGF,PLA2G7,prolactin,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3,TNF-α,TNFRI,TNFRII,VEGF etc. |
Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
Recovery | 80%-120% |
Assay Time | 2.33h |
Swiss Prot | P41246 |
Gene ID | 100008993 |
Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗兔MMP-9单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的兔MMP-9会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗兔MMP-9抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的兔MMP-9发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的兔MMP-9,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的兔MMP-9浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中兔MMP-9的浓度。 |
Background | The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines,chemokines,growth factors and binding proteins,cell/cell adhesion molecules,and other proteinases. With a few exceptions,MMPs share common structural motifs including a pro-peptide domain,a catalytic domain,a hinge region,and a hemopexin-like domain. Synthesized as pro-enzymes,most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription,pro-enzyme activation,or by their endogenous inhibitors,tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP). |
Unit | Kit |
Specification | 48T 96T |