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Determination of Significance：
Aconitase (ACO) is an important intracellular ferritin, which is mainly present in the cytoplasm and mitochondria. ACO catalyzes the reversible reaction of intracellular citric acid to isocitric acid via cis aconitic acid, which plays an important role in maintaining the success of the tricarboxylic acid cycle and the glyoxylic acid cycle.
Measurement Principle：
Aconitase can catalyze isocitrate to produce aconitic acid. Cisaconitic acid has a characteristic absorption peak at 240 nm. The enzyme activity is calculated by measuring the rate of cisaconitic acid production.
Self Provided：
Spectrophotometer/microplate reader, desk centrifuge, water bath/constant temperature incubator, adjustable transferpettor, ultrasonic cell pulverizer, micro quartz cuvette/96 well (UV) plate, mortar/homogenizer, ice and distilled water.
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