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My CartReactivity：Human,Mouse
Application：WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2737
Host：Rabbit
Qty：
Size：
| Name | Anti-GLI-3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-1:3000. |
| Target | GLI-3 |
| Background | This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. |
| Swiss Prot | P10071 |
| Gene ID | 2737 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human GLI-3 |
| Public Immunogen Range | 35-85/1580aa |
| Calculated MW | 169kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with GLI-3 antibody diluted at 1:2000;Lane: Mouse liver,Mouse liver.
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