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My Cart> Antibodies > Primary Antibodies > Anti-phospho-AKT (Ser473) Polyclonal AntibodyReactivity：Rat,Mouse,Human
Application：WB,IHC,IF,FCM,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：207
Host：Rabbit
Qty：
Size：
| Name | Anti-phospho-AKT (Ser473) Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chicken;Dog;Pig;Cow;Rabbit;Sheep |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 2ug/Test. IF 1:100-500. |
| Target | phospho-AKT |
| Background | This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. |
| Swiss Prot | P31749 |
| Gene ID | 207 |
| Subcellular Location | Cytoplasm Nucleus Cell membrane |
| Immunogen | A synthetic peptide of human AKT around the phosphorylation site of Ser473 |
| Calculated MW | 56kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with phospho-AKT (Ser473) antibody diluted at 1:300;Lane: Mouse Heart.
Immunohistochemistry of paraffin-embedded Human breast with phospho-AKT antibody diluted at 1:400
Immunohistochemistry of paraffin-embedded human breast with phospho-AKT/PKB(Ser473) antibody diluted at 1:500.
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