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CAS:5986-55-0
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:GC≥98%
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Examples of using this product（for reference only）
In Vitro:
Cell（H9C2 cells；0-1000 μmol/L Patchouli alcohol/PA；8 h）：
H9C2 cells were treated with 0, 0.1, 1, 10, 100 and 1000 μmol/L PA for 8 h, in the presence or absence of SI/R treatment and then cultured in 96-well plates at 37 ℃ with 5% CO2. Then, 10 μL CCK-8 reagent was added to the plates and incubated at 37 ℃ for 4 h. The OD value at 450 nm absorbance was recorded and determined using a microplate reader.
Reference：
Lu Y, Li SY, Lou H. Patchouli alcohol protects against myocardial ischaemia-reperfusion injury by regulating the Notch1/Hes1 pathway. Pharm Biol. 2022 Dec;60(1):949-957. doi: 10.1080/13880209.2022.2064881.PMID: 35588098;PMCID: PMC9122376.
In Vivo:
Mice（C57BL/6 male mice；10-40 mg/kg Patchouli alcohol/PA, I.P)：
The mice were randomly divided into five groups (n = 6 per group): the sham group, MI/R group, MI/R t PA-10 group, MI/R t PA-20 group and MI/R t PA-40 group. The MI/R model was established according to the research of Zhang et al. Briefly, after anaesthetisation with 1% sodium pentobarbital, the chest wall of the mice was cut open, and the left anterior descending coronary artery (LAD) was ligated with 6-0 silk thread. After 30 min of ischaemia, the slip knot was released for reperfusion injury. Mice in the sham operation group did not have LAD ligation, and the other procedures were the same as those in the MI/R group. The mice in the treatment groups were intraperitoneally injected with PA (10, 20 and 40 mg/kg) for 30 days before surgery.
Reference：
Lu Y, Li SY, Lou H. Patchouli alcohol protects against myocardial ischaemia-reperfusion injury by regulating the Notch1/Hes1 pathway. Pharm Biol. 2022 Dec;60(1):949-957. doi: 10.1080/13880209.2022.2064881.PMID: 35588098;PMCID: PMC9122376.
