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CAS:16561-29-8
Appearance:White to yellow solid
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro:
Cell（THP-1 cells；100 ng/mL PMA；24h）:
The human monocytic cell line (THP-1) was cultured in RPMI medium 1640 supplemented with 10% FBS and 50 μg/mL penicillin/streptomycin in the incubator at 37℃ with 5% CO2 balanced with air. THP-1 monocytic cells were treated with 100 ng/mL phorbol myristate acetate (PMA) (Solarbio, Beijing, China) for 24 h to induce macrophage phenotype.
Reference：
Lai D, Zhu K, Li S, Xiao Y, Xu Q, Sun Y, Yao P, Ma D, Shu Q. SARS-CoV-2 N Protein Triggers Acute Lung Injury via Modulating Macrophage Activation and Infiltration in In Vitro
and In Vivo. J Inflamm Res. 2023 Apr 28;16:1867-1877. doi: 10.2147/JIR.S405722. PMID: 37143821; PMCID: PMC10153437.
Cell（the peripheral blood lymphocytes, 50 ng/ml PMA, 5h）:
Blood from the M6-immunized (three doses) mice or rhesus monkeys was collected at and 28 days post-infection. Red blood cells were removed, and the peripheral blood lymphocytes were washed and suspended in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin. Then, the cells were cultured for 5 h in the presence of phorbol 12-myristate 13-acetate (PMA) (50 ng/ml;Solarbio),ionomycin calcium salt (1 μg/ml; Solarbio) and GolgiStop (0.67 μl/ml)
Reference：
Xu X, Feng X, Wang L, Yi T, Zheng L, Jiang G, Fan S, Liao Y, Feng M, Zhang Y, Li D, Li Q. A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect. PLoS Pathog. 2020 Aug 10;16(8):e1008703. doi: 10.1371/journal.ppat.1008703. PMID: 32776994; PMCID: PMC7440667.
Cell（THP-1 cell，200 ng/mL PMA，24h）:
THP-1 cells (1.5×105 cells/ml) were treated with 200 ng/ml phorbol myristate acetate (PMA, Solarbio Science & Technology Co., Ltd, Beijing, China.) for 24 h and polarized into macrophages. To induce TAMs, THP-1 macrophages were cultured with CM of GC cells for a further 24h prior to harvesting.
Reference：
Sun L , Li J , Yan W, et al. H19 contributes to aerobic glycolysis, proliferation and immune escape of gastric cancer cells via the miR-519d-3p/LDHA axis. 2020.
Cell（THP-1 AR silencing and differentiation，50 ng/mL PMA，24h）:
THP-1 cells were infected for 24 h with lentiviruses harboring (AR scramble or AR shRNA) shRNAs that target human AR and cloned into the U6-shRNA-EF1α- EGFP-Puro vector. The U6-shRNA-EF1α- EGFP-Puro vector encoding a scrambled sequence not matching any mammalian sequence was used as control. Positively infected cells were sorted by flow cytometry 72 h after infection. Infected THP-1 monocytes (THP-1sc and THP-1ARsi) were induced to differentiate into macrophages (MФsc and MФARsi) by exposing to 50 ng/mL phorbol myristate acetate (PMA) (Solarbio, Beijing, China) for 24 h, then incubated for an additional 24 h in the absence of PMA in a conditioning medium (CM). The collected CM was used to treat HASMCs for investigating the role of AR in HASMC calcification.
Reference：
Pang H, Xiao L, Lu Z, Chen H, Shang Z, Jiang N, Wang X, Wei F, Jiang A, Chen Y, Niu Y. Targeting androgen receptor in macrophages inhibits phosphate-induced vascular smooth muscle cell calcification by decreasing IL-6 expression. Vascul Pharmacol. 2020 Jul;130:106681. doi: 10.1016/j.vph.2020.106681. Epub 2020 May 5. PMID: 32387336.
Cell（HepG2 cells，10 nmol/L PMA，1h，37 ℃）:
To monitor endogenous ClO?, HepG2 cells were treated with LPS(1 μg/mL) for 12 h and then coincubated with PMA (10 nmol/L) and the peptide@Ag/Au NCs solution at 37 ℃ for 1 h, followed by washing three times before imaging. In the control assay, HepG2 cells were treated with LPS (1 μg/mL) for 12 h and then incubated with PMA (10 nmol/L) for 1 h. The cells were subsequently cultured in medium containing uric acid (250 nmol/L) and DMSO (0.5%) for 15 min and then treated with the peptide@Ag/Au NCs solution at 37 ℃ for 1 h. The cells were washed three times with PBS to remove the unbound peptide@Ag/Au NCs and were then observed under a Nikon A1R MP multiphoton microscope with a 60× oil-immersion objective lens. The images of peptide@Ag/Au NCs were captured under excitation at409 nm.
Reference：
Jia M, Mi W, Guo S, Yang QZ, Jin Y, Shao N. Peptide-capped functionalized Ag/Au bimetal nanoclusters with enhanced red fluorescence for lysosome-targeted imaging of hypochlorite in living cells. Talanta. 2020 Aug 15;216:120926. doi: 10.1016/j.talanta.2020.120926. Epub 2020 Mar 14. PMID: 32456892.
