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My CartCAS：99-20-7
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
Qty：
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| CAS | 99-20-7 |
| Name | Trehalose |
| Molecular Formula | C12H22O11 |
| Molecular Weight | 342.3 |
| Solubility | Soluble in Water ≥5mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 202-739-6 |
| MDL | MFCD00006628 |
| SMILES | O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@H]1O[C@@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O |
| InChIKey | HDTRYLNUVZCQOY-LIZSDCNHSA-N |
| InChI | InChI=1S/C12H22O11/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12/h3-20H,1-2H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-/m1/s1 |
| PubChem CID | 7427 |
| Background | Commonly used as additives and pharmaceutical excipients. |
| Unit | Bottle |
| Specification | 20mg 10mM*1mL in Water 50mg |
Examples of using this product（for reference only）
In Vitro:
Cell（Adipose-derived stem cells/ADSCs; 0.3 M ~1.25 M Trehalose）：
Cryoprotective agents(CPAs) preparationFor the CPA preparation, we used the following methods: (i) trehalose (Tre) group: trehalose powder (Solarbio, China) was diluted with phosphate buffer saline (PBS) for the powder reconstitution according to the required concentrations (0.3 M Tre, 0.6 M Tre, 1.0M Tre, and 1.25 M Tre) and filtered using a 0.22-μm filter; (ii) glycerol (Gly) group: glycerol was diluted with PBS to yield the required concentration (10% Gly, 20% Gly, and 30% Gly); (iii) trehalose and glycerin group: 1.0 M Tre + 20% Gly, as previously described, trehalose powder was diluted with PBS and then diluted with glycerol according to our needs; (iv) 10% DMSO + 90% FBS as a positive control; and (v) control group: only PBS without trehalose and glyceron as a negative control. At least 30 ml of the CPA (30 vials) was preserved per group.
Reference：
Zhang TY, Tan PC, Xie Y, Zhang XJ, Zhang PQ, Gao YM, Zhou SB, Li QF. The combination of trehalose and glycerol: an effective and non-toxic recipe for cryopreservation of human adipose-derived stem cells. Stem Cell Res Ther. 2020 Oct 31;11(1):460. doi: 10.1186/s13287-020-01969-0. PMID: 33129347; PMCID: PMC7602354.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Zhang TY, et al. Stem Cell Res Ther. 2020 Oct 31;11(1):460.
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