CAS:99-20-7
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
Examples of using this product(for reference only)
In Vitro:
Cell(Adipose-derived stem cells/ADSCs; 0.3 M ~1.25 M Trehalose):
Cryoprotective agents(CPAs) preparationFor the CPA preparation, we used the following methods: (i) trehalose (Tre) group: trehalose powder (Solarbio, China) was diluted with phosphate buffer saline (PBS) for the powder reconstitution according to the required concentrations (0.3 M Tre, 0.6 M Tre, 1.0M Tre, and 1.25 M Tre) and filtered using a 0.22-μm filter; (ii) glycerol (Gly) group: glycerol was diluted with PBS to yield the required concentration (10% Gly, 20% Gly, and 30% Gly); (iii) trehalose and glycerin group: 1.0 M Tre + 20% Gly, as previously described, trehalose powder was diluted with PBS and then diluted with glycerol according to our needs; (iv) 10% DMSO + 90% FBS as a positive control; and (v) control group: only PBS without trehalose and glyceron as a negative control. At least 30 ml of the CPA (30 vials) was preserved per group.
Reference:
Zhang TY, Tan PC, Xie Y, Zhang XJ, Zhang PQ, Gao YM, Zhou SB, Li QF. The combination of trehalose and glycerol: an effective and non-toxic recipe for cryopreservation of human adipose-derived stem cells. Stem Cell Res Ther. 2020 Oct 31;11(1):460. doi: 10.1186/s13287-020-01969-0. PMID: 33129347; PMCID: PMC7602354.