| CAS |
19130-96-2 |
| Chinese Name |
脱氧野尻霉素 |
| English Name |
1-Deoxynojirimycin |
| Synonyms |
1-脱氧野尻霉素;(2R，3R，4R，5S)-2-羟甲基哌啶-3，4，5-三醇;DNJ; |
| Molecular Formula |
C6H13NO4 |
| Molecular Weight |
163.17 |
| Solubility |
Soluble in Water |
| Purity |
HPLC≥98% |
| Appearance |
White to off-white Solid |
| Storage |
Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC |
EINECS 606-239-2 |
| MDL |
MFCD00063474 |
| SMILES |
O[C@@H]1[C@@H](CO)NC[C@H](O)[C@H]1O |
| InChIKey |
LXBIFEVIBLOUGU-JGWLITMVSA-N |
| InChI |
InChI=1S/C6H13NO4/c8-2-3-5(10)6(11)4(9)1-7-3/h3-11H,1-2H2/t3-,4+,5-,6-/m1/s1 |
| PubChem CID |
29435 |
| Target Point |
α-glucosidase |
| Passage |
Metabolic Enzyme&Protease |
| Background |
It is a potent α-glucosidase inhibitor. |
| Biological Activity |
1-Deoxynojirimycin (Duvoglustat) is a potent and orally active α-glucosidase inhibitor. 1-Deoxynojirimycin suppresses postprandial blood glucose and is widely used for diabetes mellitus. 1-Deoxynojirimycin possesses antihyperglycemic, anti-obesity, and antiviral features[1][2]. |
| In Vitro |
1-Deoxynojirimycin(DNJ)up to 200 μmol/L does not influence survival of HUVECs. DNJ behaves as an antioxidant，decreases ROS production，and delays cellular senescence[4]. |
| In Vivo |
1-Deoxynojirimycin(Duvoglustat)(20-80 mg/kg; i.v.; daily for four weeks)shows anti-obesity effect .1-Deoxynojirimycin significantly improves insulin sensitivity via activating insulin signaling PI3K/AKT pathway in skeletal muscle of db/db mice[3]. |
| Animal Experiment |
Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates(4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃，the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h，10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃，cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.[4] |
| Data Literature Source |
[1]. Gao K，et al. 1-Deoxynojirimycin: Occurrence，Extraction，Chemistry，Oral Pharmacokinetics，Biological Activities and In Silico Target Fishing. Molecules. 2016 Nov 23;21(11). pii: E1600.
[2]. Chaluntorn Vichasilp，et al. Development of high 1-deoxynojirimycin (DNJ) content mulberry tea and use of response surface methodology to optimize tea-making conditions for highest DNJ extraction. LWT - Food Science and Technology. Volume 45，Issue 2，March 2012，Pages 226-232
[3]. Liu Q，et al. 1-Deoxynojirimycin Alleviates Insulin Resistance via Activation of Insulin Signaling PI3K/AKT Pathway in Skeletal Muscle of db/db Mice. Molecules. 2015 Dec 4;20(12):21700-14.
[4]. E S，Kijima R，Honma T，Yamamoto K，Hatakeyama Y，Kitano Y，Kimura T，Nakagawa K，Miyazawa T，Tsuduki T. 1-Deoxynojirimycin attenuates high glucose-accelerated senescence in human umbilical vein endothelial cells. Exp Gerontol. 2014 Jul;55:63-9. |
| Unit |
Piece |
| Specification |
5mg 10mg |
English
中文
Manual Download
