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CAS:58-58-2
Appearance:White to light yellow solid
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro：
Cell（OC cells，2 μg/mL puromycin）:
PCNP expression plasmid and empty vector, the PCNP shRNA (sh-PCNP group) and scramble shRNA (sh-Scb group) and were transfected into OC cells with Lipofectamine 3000 Transfection Reagent to construct stable cell lines. They were screened, respectively, by G418 (Solarbio, Shanghai, China) at a concentration of 800 μg/mL and puromycin (Solarbio, Shanghai, China) at a concentration of 2 μg/mL.
Reference：
Dong P, Fu H, Chen L, Zhang S, Zhang X, Li H, Wu D, Ji X. PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. J Cell Mol Med. 2020 Jul;24(14):8221-8235. doi: 10.1111/jcmm.15491. Epub 2020 Jun 16. PMID: 32548978; PMCID: PMC7348179.:
Cell（A549 and PC-9 cells，2 mg/mL puromycin）:
Lentivirus transfection:
24 h before lentivirus transfection, A549 and PC-9 cells were plated into 12-well dishes (1×105 cells/well). The original medium substituted for 1 mL of fresh medium containing polybrene and an appropriate virus suspension was added for incubation at 37℃. After 24 h, the medium was replaced with fresh medium without virus. Afterwards, cells were screened with 2 mg/mL puromycin (Solarbio, Beijing, China) to obtain stable cell lines.
Reference：
Wang R, Hou Y, Geng G, Zhu X, Wang Z, Cai W, Ye J, Zhao S, Mi Y, Jiang J. Onvansertib inhibits the proliferation and improves the cisplatin-resistance of lung adenocarcinoma via β-catenin/c-Myc signaling pathway. Am J Cancer Res. 2023 Feb 15;13(2):623-637. PMID: 36895968; PMCID: PMC9989612.
Cell（A549, H446 and 95-D cells ，2 μg/mL puromycin）：
A549, H446 and 95-D cells were infected with lentivirus in the presence of 8 μg/mL polybrene for 24 h and were selected with 2 μg/mL puromycin (Solarbio, IP1280, Beijing, China) for two weeks.
Reference：
Zeng C, Zou T, Qu J, Chen X, Zhang S, Lin Z. Cyclovirobuxine D Induced-Mitophagy through the p65/BNIP3/LC3 Axis Potentiates Its Apoptosis-Inducing Effects in Lung Cancer Cells. Int J Mol Sci. 2021 May 29;22(11):5820. doi: 10.3390/ijms22115820. PMID: 34072333; PMCID: PMC8199090.
Cell（H69-AR cells, 1.5 μg/ml puromycin ）：
Shortly, 3×107 H69-AR cells per sample were infected by the lentivirus to achieved 250-fold coverage at an MOI of 0.3. Then the cells were selected with 1.5 μg/ml puromycin (Solarbio, China) for 72 h.
Reference：
Deng L, Yang L, Zhu S, Li M, Wang Y, Cao X, Wang Q, Guo L. Identifying CDC7 as a synergistic target of chemotherapy in resistant small-cell lung cancer via CRISPR/Cas9 screening. Cell Death Discov. 2023 Feb 2;9(1):40. doi: 10.1038/s41420-023-01315-2. PMID: 36725843; PMCID: PMC9892530.
