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Your Position: Home > Small Molecule Compounds > Inhibitors & Antagonists & Agonists > Metabolic Enzyme & Protease > Cysteine Protease > E-64
E-64

CAS:66701-25-5

Appearance:White to off-white solid

Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months

Purity:≥98%

   Manual Download
E-64
Cat.No：
IE0610
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A


Examples of using this product（for reference only）

In Vitro:

Western blotting. （14 μM E-64）:

MCF-7 cells were treated with the aforementioned CoCl2 concentrations for 24 h and cells were lysed with ice-cold radioimmunoprecipitation assay buffer (150 mM NaCl, 1.0% NP-40, 0.1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS and 50 mM Tris-HCl, pH 8.0) and protease inhibitors (AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China).

Reference：

Li Q, Ma R, Zhang M. CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells. Oncol Lett. 2018 Jan;15(1):1119-1124. doi: 10.3892/ol.2017.7369. Epub 2017 Nov 8. PMID: 29391899; PMCID: PMC5769392.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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