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My CartApplication：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：7.8 pg/ml
Storage：2-8℃，6 months
Gene ID：3273
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| Name | Human HRG ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | HRG |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.62-1000 pg/ml |
| Sensitivity | 7.8 pg/ml |
| Specificity | Do not react with Human HRG analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P04196 |
| Gene ID | 3273 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人HRG 单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人HRG 会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人HRG 抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人HRG 发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人HRG ，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人HRG 浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人HRG 的浓度。 |
| Background | Histidine-rich glycoprotein，also known as HRG and HPRG，is a glycoprotein located in plasma and platelets，and contains an unusually large amount of histidine and proline. In human，five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 35 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 1 tandem repeats with an HHPHG motif，and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear，but it is known that the protein binds heme，dyes and divalent metal ions. It inhibits rosette formation and interacts with heparin，thrombospondin and plasminogen. Two of the protein's effects，the inhibition of fibrinolysis and the reduction of inhibition of coagulation，indicate a potential prothrombotic effect. HPRG is evolutionarily，functionally and structurally related to cleaved high molecular weight kininogen (HKa)，an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated，at least partially，through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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