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My CartReactivity：Human
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：11319
Host：Rabbit
Qty：
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| Name | Anti-ECD Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:25-100. |
| Target | ECD |
| Background | Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes . Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins . The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex . May play a role in regulation of pre-mRNA splicing . |
| Swiss Prot | O95905 |
| Gene ID | 11319 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human ECD |
| Public Immunogen Range | 295-644aa |
| Calculated MW | 68/73/78kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ECD antibody diluted at 1:2000;Lane: PC-3M-IE8,MCF7,HeLa,293T.
Immunohistochemistry of paraffin-embedded Human prostate cancer with ECD antibody diluted at 1:50.
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