Conventional Tris-SDS-PAGE electrophoresis can only distinguish large molecular proteins, and the resolution is very low for relatively small molecular weights, especially for proteins below 10 kD. Tricine-SDS-PAGE can separate proteins and peptides with molecular weight in the range of 1-10 kD, which has become the main method of electrophoresis denaturation and separation of peptides. This product includes a complete set of reagents required for the preparation of Tricine-SDS-PAGE gel. Only distilled water is needed to prepare high-quality gel of various concentrations, which is convenient and fast. After electrophoresis, it can be directly used for testing, silver dyeing, Western hybridization and other experiments.

The matching protein Marker of this product consists of 3 kinds of predyed polypeptides and 2 kinds of low molecular weight proteins, with a molecular weight range of 3.3kD-31.0kD, which can be used to directly observe the protein electrophoresis status and clearly judge the membrane transfer effect of Western Blot. 5 clear blue protein bands can be seen during Tricine glycerol-SDS-PAGE gel electrophoresis and when transferred to PVDF or NC film. This product is a freeze-dried powder of protein and polypeptide mixture with approximately 40μg of each predyed protein and a 1× loading buffer. The 2× loading buffer (including DTT) is suitable for protein loading in Tricine-SDS-PAGE electrophoresis. Its main components are SDS, DTT, Coomasi bright blue, buffer salt solution and so on. Coomassie brilliant Blue is used as an indicator during electrophoresis, indicating roughly when the electrophoresis will end.

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