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Ascorbic Acid / Total Ascorbic Acid (AsA/T-AsA) Assay Kit (Colorimetric)

BC4630-241212Manual

Cat.No：BC4630 Solarbio

Detection Method：Spectrophotometer

Storage：Store at -20℃，6 months

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Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	Ascorbic Acid / Total Ascorbic Acid (AsA/T-AsA) Assay Kit (Colorimetric)
Detection Method	Spectrophotometer
Storage	Store at -20℃，6 months
Unit	Kit
Specification	50T/24S

Determination of Significance：

AsA is also called Vitamin C. AsA is the substrate of coenzyme, free radical scavenger, electron copolymer/receptor, biosynthesis of oxalate and tartrate. As the most important antioxidant in plant cells, AsA has important function in protecting chloroplast from oxidizing. It is also one of the important indexes to measure the quality of crop products. DHA is a reversible oxidized form of AsA. It forms a redox system with ascorbic acid in the living body and has the function of an electron acceptor.

Measurement Principle：

ASA has reductive ability, which can reduce Fe³⁺ to Fe²⁺. Fe²⁺ and 2,2’-bipyridine form a pink complex, with a characteristic absorption peak at 525 nm. DTT can reduce DHA to generate ASA, for this can be used to detect the total ascorbic acid( ASA + DHA) content of samples.

Self Provided：

Mortar, ice, low temperature centrifuge, spectrophotometer, 1 mL glass cuvette, transferpettor, ethanol and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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