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My Cart> Biochemical Assay Kits > REDOX Series > Vitamin C Metabolism Series > Ascorbic Acid / Total Ascorbic Acid (AsA/T-AsA) Assay Kit (Colorimetric)Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Ascorbic Acid / Total Ascorbic Acid (AsA/T-AsA) Assay Kit (Colorimetric) |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Ascorbic acid (AsA) is a kind of coenzyme, free radical scavenger, electron acceptor and substrate of oxalate and tartrate biosynthesis. As the most important antioxidant in plant cells, AsA plays an important role in protecting chloroplasts from oxidative damage, and is also one of the important indicators to measure the quality of crop products. Dehydroascorbic acid (DHA) is a reversible oxidation type of AsA. In vivo, it forms an redox system with ascorbic acid and has the function of electron acceptor.
Measurement Principle：
AsA is reductive and can reduce Fe3+ to Fe2+. Fe2+ and 2,2'-bipyridine form a pink complex with a characteristic absorption peak at 525 nm. DTT can reduce DHA to produce AsA, which can be used to detect the content of total ascorbic acid (AsA+DHA) in samples.
Self Provided：
Spectrophotometer/microplate reader, low tempareture centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ethanol, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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