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Storage:Store at -20℃，6 months
Determination of Significance：
Ascorbic acid (AsA) is a kind of coenzyme, free radical scavenger, electron acceptor and substrate of oxalate and tartrate biosynthesis. As the most important antioxidant in plant cells, AsA plays an important role in protecting chloroplasts from oxidative damage, and is also one of the important indicators to measure the quality of crop products. Dehydroascorbic acid (DHA) is a reversible oxidation type of AsA. In vivo, it forms an redox system with ascorbic acid and has the function of electron acceptor.
Measurement Principle：
AsA is reductive and can reduce Fe3+ to Fe2+. Fe2+ and 2,2'-bipyridine form a pink complex with a characteristic absorption peak at 525 nm. DTT can reduce DHA to produce AsA, which can be used to detect the content of total ascorbic acid (AsA+DHA) in samples.
Self Provided：
Spectrophotometer/microplate reader, low tempareture centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ethanol, ice and distilled water.
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