CAS |
599-79-1 |
Chinese Name |
柳氮磺吡啶 |
English Name |
Sulfasalazine |
Synonyms |
Azulfidine;NSC 667219 |
Molecular Formula |
C18H14N4O5S |
Molecular Weight |
398.39 |
Solubility |
Soluble in DMSO ≥5mg/mL |
Purity |
≥98% |
Appearance |
Light yellow to orange Solid |
Storage |
Powder:2-8℃,2 years;Insolvent(Mother Liquid):-20℃,6 months;-80℃,1 year |
EC |
EINECS 209-974-3 |
MDL |
MFCD00057363 |
SMILES |
OC(C1=C(O)C=CC(/N=N/C2=CC=C(S(=O)(NC3=NC=CC=C3)=O)C=C2)=C1)=O |
InChIKey |
NCEXYHBECQHGNR-UHFFFAOYSA-N |
InChI |
InChI=1S/C18H14N4O5S/c23-16-9-6-13(11-15(16)18(24)25)21-20-12-4-7-14(8-5-12)28(26,27)22-17-3-1-2-10-19-17/h1-11,23H,(H,19,22)(H,24,25) |
PubChem CID |
5339 |
Target Point |
NF-κB; Autophagy; Apoptosis; Ferroptosis |
Passage |
NF-κB; Autophagy; Apoptosis |
Background |
Sulfasalazine is a potent, specific inhibitor of nuclear factor kappa B (NF-κB). Sulfasalazin can induce iron death, apoptosis and autophagy. |
Biological Activity |
Sulfasalazine是治疗类风湿关节炎和溃疡性结肠炎的药物。报道显示Sulfasalazine可抑制 NF-κB 的活性。[1-3] |
In Vitro |
用柳氮磺胺吡啶处理SW620结肠细胞抑制TNFα-,LPS-或佛波醇酯诱导的NFκB活化。在微量至毫摩尔浓度下,柳氮磺胺吡啶可抑制NFκB依赖性转录。通过抑制IκBα降解,柳氮磺胺吡啶可以预防TNFα诱导的NFκB核转位[1]。与载体对照相比,单独用5mM柳氮磺吡啶预孵育显着增加所有促炎细胞因子的基础mRNA表达,IL-6 mRNA水平增加80倍[2]。一旦被消化,柳氮磺胺吡啶被结肠细菌切割成磺胺吡啶和5-氨基水杨酸,后者也被报道抑制NF-κB活性[3]。 |
In Vivo |
在低剂量(0.25 mM)下,与未经治疗的对照相比,SAS能够抑制胶质瘤生长超过60%[3]。 |
Cell Experiment |
柳氮磺胺吡啶溶解在培养基中。 SW620细胞在Dulbecco改良的Eagle培养基中生长,补充有10%热灭活的FCS,2mmol /升谷氨酰胺和1%(wt / vol)青霉素/链霉素。用3xIgkBLuc报道构建体转染SW620细胞。 18小时后,在用TNFα,LPS或PMA刺激之前,将细胞与单独的培养基或柳氮磺胺吡啶(0.1,0.2,0.5,1,2,5mM)一起温育。进行荧光素酶测定[1]。 |
Animal Experiment |
小鼠:将柳氮磺胺吡啶溶于0.1M NaOH中,然后用0.1M HCl滴定中和。将U-87MG神经胶质瘤细胞植入SCID小鼠的颅骨中。 7天后,将动物随机分成三组,每组五只动物。一组每天两次接受1mL ip盐水注射,持续3周。两个试验组每天两次在1mL盐水中接受8mg柳氮磺吡啶,持续3周。监测肿瘤生长和动物健康。用4%多聚甲醛灌注后,收集小鼠脑,冲洗,并置于30%蔗糖中[3]。 |
Data Literature Source |
[1]. Wahl C, et al. Sulfasalazine: a potent and specific inhibitor of nuclear factor kappa B. J Clin Invest. 1998 Mar 1; 101 (5) :1163-74. [2]. Sykes L, et al. Sulfasalazine augments a pro-inflammatory response in interleukin-1β-stimulated amniocytes and myocytes. Immunology. 2015 Dec; 146 (4) :630-44. [3]. Chung WJ, et al. Sulfasalazine inhibits the growth of primary brain tumors independent of nuclear factor-kappaB. J Neurochem. 2009 Jul; 110 (1) :182-93 |
Unit |
Bottle |
Specification |
500mg 10mM*1mL in DMSO 1g |