Products
DPPH

CAS:1898-66-4

Storage:Powder:2-8℃,2 years;Insolvent(Mother Liquid):-20℃,6 months;-80℃,1 year

Purity:HPLC≥98%

Appearance:Black Solid

DPPH
Cat.No:
ID1240
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Examples of using this product(for reference only)

DPPH scavenging activities:

The DPPH scavenging activities (%) of fresh MPN-GA samples (CON, A1, A2, F1 and F2) were evaluated via using the methods of Zhang et al. [17]. To be specific, fresh MPN-GAs (2.0 mL, 0, 0.01, 0.05, 0.25, 1 and 4 mg/mL), DPPH ethanol solution (0.8 mL, 0.4 mM) were incorporated into the water (4.0 mL). Then, obtained mixtures were centrifuged (6000 rpm, 15 min) after the reaction for 30 min (4 ℃, in the darkness). Final DPPH scavenging activities were assessed through the transformation of measured absorbance (517 nm).

Reference:

Chen J, Chen X, Zhou G, Xu X. New insights into the ultrasound impact on covalent reactions of myofibrillar protein. Ultrason Sonochem. 2022 Mar;84:105973. doi: 10.1016/j.ultsonch.2022.105973. Epub 2022 Mar 3. PMID: 35272240; PMCID: PMC8913343.

Antioxidant capacity:

Film samples (4–20 mg) were added to 10 mL of distilled water and stirred until completely dissolved. DPPH solution (0.2 mmol/L) was prepared in anhydrous ethanol (≥ 99.7 %). Film solution (2 mL) was mixed with 2 mL of DPPH solution and the absorbance at 517 nm measured after reacting for 30 min at 25 ℃ in the dark. The DPPH radical scavenging rate of a Vit C solution at the same concentration as the film solution was determined as a positive control. The DPPH radical scavenging activity of film samples was calculated as follows: DPPH radical scavenging activity (%) = (A0 ? A1 + A2)/ A0×100

where A0 is the absorbance of the mixture of distilled water and DPPH solution, A1 is the absorbance of the mixture of film or Vit C solution and DPPH solution, and A2 is the absorbance of the mixture of anhydrous alcohol and film or Vit C solution.

Reference:

Liu Z, Du M, Liu H, et al. Chitosan films incorporating litchi peel extract and titanium dioxide nanoparticles and their application as coatings on watercored apples[J]. Progress in Organic Coatings, 2021, 151:106103.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
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