DPPH
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Cat.No：ID1240 Solarbio
CAS：1898-66-4
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：Black Solid
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> Small Molecule Compounds > Reaction Substrate & Physicochemical Detection > DPPH
Product Information
| CAS | 1898-66-4 |
| Name | DPPH |
| Molecular Formula | C18H12N5O6 |
| Molecular Weight | 394.32 |
| Solubility | Soluble in DMSO ≥20mg/mL; Soluble in Methanol ≥2mg/mL;Soluble in Ethanol ≥0.01mg/mL |
| Purity | HPLC≥98% |
| Appearance | Black Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 217-591-8 |
| MDL | MFCD00007231 |
| SMILES | O=[N+](C1=C([N]N(C2=CC=CC=C2)C3=CC=CC=C3)C([N+]([O-])=O)=CC([N+]([O-])=O)=C1)[O-] |
| Target Point | Others |
| Passage | Others |
| Background | DPPH is a stable free radical that can be used to measure the free radical scavenging activity of antioxidants. The DPPH radical has a single electron， its alcohol solution is purple， and has a strong absorption at 517nm. When free radical scavenger is present， its absorption gradually disappears due to its single electron pairing， and its degree of fading is quantitatively related to the number of electrons it accepts， so it can be used for rapid quantitative analysis by spectrophotometer. |
| Biological Activity | DPPH是一种稳定的自由基，可用于测量抗氧化剂的自由基清除活性。DPPH 中氮原子的奇数电子通过从抗氧化剂吸收氢原子而还原为相应的肼。DPPH 方法可用于水性和非极性有机溶剂中，并可用于检查亲水性和亲脂性抗氧化剂[1]。 |
| In Vitro | DPPH由于其奇数电子而在517nm处显示出强吸收带，并且溶液呈现深紫色，当电子对断开时吸收消失。得到的脱色相对于吸收的电子数是化学计量的。0.5 mM的酒精溶液颜色密集，在此浓度下，在有用的吸收范围内遵守Lambert-Beer定律[1]。DPPH测定是一种快速，简单，廉价且广泛使用的方法，用于测量化合物作为自由基清除剂或氢供体的能力，并评估食物的抗氧化活性。它还可用于量化复杂生物系统中的抗氧化剂，固体或液体样品。该方法简便，适用于测定果汁和蔬菜汁的总抗氧化能力和自由基清除活性。该试验已成功用于研究小麦籽粒和麸皮，蔬菜，共轭亚油酸，草药，食用种子油和面粉在几种不同溶剂体系中的抗氧化性能，包括乙醇，丙酮水溶液，甲醇，乙醇水溶液和苯。它是一种方便的方法，用于橄榄油，水果，果汁和葡萄酒中半胱氨酸，谷胱甘肽，抗坏血酸，生育酚和多羟基芳香族化合物的抗氧化测定[1]。 |
| Data Literature Source | [1]. Kedare SB，et al. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011 Aug;48(4):412-22. |
| Unit | Bottle |
| Specification | 5mg 10mM*1mL in DMSO 10mg 50mg |
Examples of using this product（for reference only）
DPPH scavenging activities：
The DPPH scavenging activities (%) of fresh MPN-GA samples (CON, A1, A2, F1 and F2) were evaluated via using the methods of Zhang et al. [17]. To be specific, fresh MPN-GAs (2.0 mL, 0, 0.01, 0.05, 0.25, 1 and 4 mg/mL), DPPH ethanol solution (0.8 mL, 0.4 mM) were incorporated into the water (4.0 mL). Then, obtained mixtures were centrifuged (6000 rpm, 15 min) after the reaction for 30 min (4 ℃, in the darkness). Final DPPH scavenging activities were assessed through the transformation of measured absorbance (517 nm).
Reference：
Chen J, Chen X, Zhou G, Xu X. New insights into the ultrasound impact on covalent reactions of myofibrillar protein. Ultrason Sonochem. 2022 Mar;84:105973. doi: 10.1016/j.ultsonch.2022.105973. Epub 2022 Mar 3. PMID: 35272240; PMCID: PMC8913343.
Antioxidant capacity：
Film samples (4–20 mg) were added to 10 mL of distilled water and stirred until completely dissolved. DPPH solution (0.2 mmol/L) was prepared in anhydrous ethanol (≥ 99.7 %). Film solution (2 mL) was mixed with 2 mL of DPPH solution and the absorbance at 517 nm measured after reacting for 30 min at 25 ℃ in the dark. The DPPH radical scavenging rate of a Vit C solution at the same concentration as the film solution was determined as a positive control. The DPPH radical scavenging activity of film samples was calculated as follows: DPPH radical scavenging activity (%) = (A0 ? A1 + A2)/ A0×100
where A0 is the absorbance of the mixture of distilled water and DPPH solution, A1 is the absorbance of the mixture of film or Vit C solution and DPPH solution, and A2 is the absorbance of the mixture of anhydrous alcohol and film or Vit C solution.
Reference：
Liu Z, Du M, Liu H, et al. Chitosan films incorporating litchi peel extract and titanium dioxide nanoparticles and their application as coatings on watercored apples[J]. Progress in Organic Coatings, 2021, 151:106103.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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