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Determination of Significance：
α-L-Arabinofuranosidase (α-L-Af) belongs to the family of glycosyl hydrolases, which can be hydrolyzed from the non-reducing ends of polymers containing arabinose residues such as arabinoxylan, arabinan and arabinose galactosan etc. to form an L-arabinose molecule. This enzyme plays an important role in the degradation of hemicellulose, the ripening and softening of fruits.
Measurement Principle：
α-L-Af decomposes p-nitrophenyl-α-L-arabinofuranoside to produce p-nitrophenol, p-nitrophenol has a maximum absorption peak at 400 nm, which can be calculated by measuring the change in absorbance at 400 nm.
Self Provided：
Spectrophotometer/ Microplate reader, cryogenic centrifuge, centrifuge, water-bath/Constant temperature incubator, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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