Human NLRP3/NALP3/CIAS1/PYPAF ELISA Kit_Human_ELISA_Solarbio-Global Leader in Antibody and Elisa Kit

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SKU	Tongzhou Beijing	Haidian Beijing	Wuhan	Guangzhou
SEKH-0504-96T	Inquiry	Inquiry	Inquiry	Inquiry
SEKH-0504-48T	Inquiry	Inquiry	Inquiry	Inquiry

Chinese Name	人核苷酸结合寡聚化结构域样受体蛋白3检测试剂盒
English Name	Human NLRP3/NALP3/CIAS1/PYPAF ELISA Kit
Synonyms	AGTAVPRL; AVP; C1orf7; CIAS1; Cryopyrin; FCAS; FCU; Muckle-Wells syndrome; NALP3; PYRIN-containing APAF1-like protein 1; Nucleotide-Binding Ologomerization Domain-Like Receptor Protein 3
Storage	2-8℃，6 months
Detection Target	NLRP3/NALP3/CIAS1/PYPAF
Sample Type	Serum，Plasma，Cell Culture Supernatant
Reactivity	Human
Application	ELISA
Reaction Type	Sandwich-ELISA
Quantitative/Qualitative	Quantitative
Detection Range	156.25-10000 pg/ml
Sensitivity	30 pg/ml
Specificity	Do not react with Human BMP-1， Human BMP-2， Human BMP-3， Human BMP-4， Human IL-1β， Human IL-4， Human IL-5， Human IL-6， Human IL-8， Human IFN-γ， Human TGF-β1， Human TGF-β2， Human TGF-β3， Human TLR1， Human TLR2， Human TLR3 etc.
Precision	Both intra-plate and inter-plate variation coefficients were less than 10%
Recovery	80%-120%
Assay Time	3.5h
Swiss Prot	Q96P20
Gene ID	114548
Detection Principle	Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人NLRP3单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人NLRP3会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人NLRP3抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人NLRP3发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人NLRP3，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人NLRP3浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人NLRP3的浓度。
Background	NLRP3，also known as NALP3，CIAS1，PYPAF or Cryopyrin，is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes，especially neutrophils. As a component of the inflammasome，NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1，CINCA，or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532)，and seven LRRs (aa 739-988). Within the sequence used as an immunogen，mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3，respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3，LRR 6 and 7，or LRR4-9.
Unit	Kit
Specification	48T 96T