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My CartReactivity：Human
Application：WB,IHC,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：57721
Host：Rabbit
Qty：
Size：
| Name | Anti-METTL14 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | METTL14 |
| Background | The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis . In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core . N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). |
| Swiss Prot | Q9HCE5 |
| Gene ID | 57721 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human METTL14 |
| Public Immunogen Range | 159-456aa |
| Calculated MW | 52kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. ELISA 1:3000-30000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB IHC ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with METTL14 antibody diluted at 1:4000;Lane: Jurkat,K562.
Immunohistochemistry of paraffin-embedded Human prostate cancer with METTL14 antibody diluted at 1:50.
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