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CAS:2016-88-8
Appearance:Light yellow to yellow solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:≥98%
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Examples of using this product（for reference only）
Cell （200 μM Amiloride，30 min）:
To investigate the endocytosis mechanism involved in the uptake process, cells were pre-treated with endocytic inhibitors including chlorpromazine (100 μM), genistein (200 μM), and amiloride (200 μM) for 30 min. Ten the cells were treated with 1 μg of FITC-labeled DMSNs for 6 h. In a control group, cells were not treated with the inhibitors prior to nanoparticle treatment. Cellular uptakes of nanoparticles were quantitated by fow cytometer as described above.
Reference：
Mo C, Lu L, Liu D, Wei K. Development of erianin-loaded dendritic mesoporous silica nanospheres with pro-apoptotic effects and enhanced topical delivery. J Nanobiotechnology. 2020 Mar 30;18(1):55. doi: 10.1186/s12951-020-00608-3. PMID: 32228604; PMCID: PMC7104482.
Cell（A549 cells and MDCK cells；50 μg/mL Amiloride）:
To investigate the uptake mechanism of virus-laden AFPs, cells were pretreated with NA enzyme (1 U/ml) at 37℃ for 2 hours and immediately incubated with diverse inhibitors in complete cell culture medium at 37℃ for 1 hour, followed by infection with virus-laden AFPs (50 μg/ml) for 24 hours. Chlorpromazine (5 μg/ ml; Solarbio, China) was used to inhibit clathrin-mediated endocytosis;methyl-β-cyclodextrin (20 μg/m) was used to inhibit lipid raft–mediated endocytosis; amiloride (50 μg/ml; Solarbio, China) was used to block micropinocytosis; cytochalasin D (2.5 μg/ml) was used to inhibit actin polymerization and macropinocytosis, as established previously.
Reference：
Dong Z, Ma J, Qiu J, Ren Q, Shan Q, Duan X, Li G, Zuo YY, Qi Y, Liu Y, Liu G, Lynch I, Fang M, Liu S. Airborne fine particles drive H1N1 viruses deep into the lower respiratory tract and distant organs. Sci Adv. 2023 Jun 9;9(23):eadf2165. doi: 10.1126/sciadv.adf2165. Epub 2023 Jun 9. PMID: 37294770; PMCID: PMC10256160.
