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OverExpressC43(DE3) strain was suitable for the expression of toxic proteins and was derived from OverExpressC41(DE3) strain. OverExpress C41(DE3) is a mutant strain obtained through screening experiment of toxic protein overexpression in BL21(DE3). The gene mutation of this strain reduces the enzyme activity of T7 RNA polymerase and down-regulates the expression of toxic protein to ensure cell survival. OverExpress C43(DE3) strain was obtained by repeated toxicity tolerance test on OverExpress C41(DE3) strain with another toxic protein. The OverExpress C43(DE3) strain was more resistant to toxic proteins than OverExpress C41(DE3). OverExpress C43(DE3) strain can also express conventional proteins, and the protein expression level is slightly lower than that of BL21(DE3) strain under the same conditions. OverExpressC43(DE3) belongs to B strains, lon and ompT protease deficient. OverExpress C43(DE3) receptor cells are made by a special process, and the conversion efficiency of pUC19 plasmid detection can reach 1×107 cfu/μg DNA.
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