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TG1 strain is derived from Escherichia coli K-12 and is mainly used for Phage Display, M13 phage-related experiments, and cloning and extraction of common plasmids. The presence of lacZΔM15 can be used for blue-white spot screening experiments on the principle of alpha complementation. Because the strain does not contain the mutation of endA1 nuclease, the content of nuclease in the extracted plasmid is high, and it needs to be treated with protein removal solution. TG1 receptor cells were made by a special process and the conversion efficiency was as high as 108cfu/μg DNA detected by pUC19 plasmid.
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Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
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