Dh5α-T1 was an improved strain of conventional DH5α. Deletion of nuclease (endA1) gene improves plasmid yield and quality; Recombinase defect type (recA1) reduces the homologous recombination probability of inserted fragments and ensures the stability of inserted DNA. Due to the presence of lacZΔM15 and the deletion of lacIq gene, it is not necessary to add IPTG, but only need to add X-gal to perform the blue-white spot screening experiment based on the principle of α-complementarity. The mutation of DH5α-T1 in the tonA region of the genome enables the strain to acquire resistance to T1 and T5 phage infection. DH5α-T1 receptor cells were made by a special process, and the conversion efficiency of pUC19 plasmid was as high as 108cfu/μg DNA.
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