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Your Position: Home > Molecular Biology > Recombinant Clone Expression > Competent Cell > dam-/dcm-Competent Cell
dam-/dcm-Competent Cell

Storage:-70℃

  C1320Manual
dam-/dcm-Competent Cell
Cat.No：
C1320
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
C1320-20*100ul Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

dam-/ DCM-receptive cells produced by our company are the receptive cells obtained from E. coli dam-/ DCM-receptive cells treated by special technology. The dam-/dcm- strain was derived from Escherichia coli K12, which was a dam and dcm methylase inactivated mutant strain and was often used for de-DAM and dcm methylation treatment of plasmid DNA to eliminate the effects of dam and dcm methylation on enzyme digestion. The deletion of endA1 gene is beneficial to the production and quality of plasmid DNA. The inactivation mutation of methylase may result in the mutation of plasmid DNA in this strain, and the transformation experiment of ligand products is not recommended, only for plasmid transformation. The strain was also resistant to T1 phage infection. Conversion efficiency of pUC19 plasmid was >×106 cfu/μg DNA.

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Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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