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T7 expressing Competent Cell

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T7 expressing Competent Cell
Cat.No:
C1370
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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The T7 Express strain is a BL21 enhanced strain of Escherichia coli and a Lon and OmpT protease deficient strain, which is mainly suitable for the protein expression of prokaryotic expression vectors containing T7 promoters (such as pET, etc.), as well as non-T7 promoter expression vectors requiring Escherichia coli RNA polymerase to transcribed RNA (such as pGEX, etc.). This strain was different from BL21 (DE3) in that the T7 RNA polymerase gene was integrated in the lac operon region of the bacterial chromosome, there was no λ-pre-phage sequence in the genome, and it was resistant to T1 phage infection. The conversion efficiency of T7 receptor cells was higher than 108 cfu/μg detected by pUC19 plasmid.

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Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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