The T7 Express strain is a BL21 enhanced strain of Escherichia coli and a Lon and OmpT protease deficient strain, which is mainly suitable for the protein expression of prokaryotic expression vectors containing T7 promoters (such as pET, etc.), as well as non-T7 promoter expression vectors requiring Escherichia coli RNA polymerase to transcribed RNA (such as pGEX, etc.). This strain was different from BL21 (DE3) in that the T7 RNA polymerase gene was integrated in the lac operon region of the bacterial chromosome, there was no λ-pre-phage sequence in the genome, and it was resistant to T1 phage infection. The conversion efficiency of T7 receptor cells was higher than 108 cfu/μg detected by pUC19 plasmid.
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