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Stbl4 strain is derived from Stbl2 strain (Stbl2 is a JM109-derived strain), which is used to clone unstable sequences (such as repeat sequences, retroviral sequences, etc.) and methylated DNA sequences, and is especially suitable for constructing recombinant retroviral or lentiviral plasmids. It can be used for the construction and amplification of large plasmids, and is suitable for constructing and expanding plasmid cDNA libraries. Different from Stbl2 strain, Stbl4 strain can be screened for blue and white spots by α-complementary principle in the presence of IPTG and X-gal. The conversion efficiency of pUC19 plasmid was greater than 108 cfu/μg.
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