TBE buffer is a buffer salt solution commonly used in biology for nucleic acid electrophoresis, mainly for agarose gel electrophoresis of DNA. The main components of TBE are TRIS-Borate and EDTA, which has strong buffer capacity, is suitable for longer electrophoresis time, high resolution, and good separation effect when electrophoresis fragments less than 1kb. The boric acid content of TBE buffer can affect the efficiency of DNA recovery and the subsequent enzyme reaction, so TAE buffer is recommended for DNA fragment recovery experiments by agar-gel electrophoresis. This product is TBE buffer premixed powder, can be quickly prepared into 89mM Tris-boric, 2 mM EDTA; pH=8.2-8.4, 1×TBE buffer.

Usage:

1, each bag of powder can be prepared into 1000ml of 1× TBE buffer.

2. Deionized water or double steaming water is used as solvent when preparing.

3, take out all the powder in the bag dissolved in 800ml deionized water or distilled water, fixed volume to 1000ml, that is, 1× TBE buffer.

4, the working concentration of TBE buffer is generally 0.5×, and the 1× TBE buffer can be diluted 1 times before electrophoresis.

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