Western Transfer Buffer,1× (powder)
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Cat.No：D1061 Solarbio
Storage：Store at RT，2 years
Appearance：Powder
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> Protein and Immunity > Protein Electrophoresis > Protein Electrophoresis Reagents > Western Transfer Buffer,1× (powder)
Product Information
| Appearance | Powder |
| Storage | Store at RT，2 years |
| Unit | Bottle |
| Specification | 5*1L 10*1L |
1× electrophoretic transfer buffer is a buffer for wet and semi-dry electrophoretic transfer of film in Western blotting experiments. This product is prepared by conventional method, is premixed powder, does not contain SDS and methanol, easy to store, easy to operate.
The components of 1× electrophoretic transfer buffer were 25mM Tris and 192mM Glycine.
Usage:
1. Each bag of powder can be prepared into 1000ml 1×Western Transfer Buffer.
2. Deionized water or double steaming water is used as solvent when preparing. Prepare the methanol solution.
3. Take out all the powder in the packaging bag and dissolve it in 600ml deionized water or distilled water, set the volume to 800ml, and mix it with 200ml methanol solution before use.
4, this product does not contain SDS, protein molecular weight is large or strong hydrophobicity, in order to increase the film transfer effect, can be appropriate to add a small amount of SDS (reference working concentration of 0.025-0.1%).
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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