EdU is a thymidine analogue, and its alkyne group is rare in natural compounds. It can replace thymine (T) to permeate into the synthetic of DNA molecule during the DNA replication period. Based on the specific reaction of Apollo fluorescent dye with EdU, the replication activity of DNA can be detected directly and accurately. EdU is widely used in cell proliferation, differentiation, growth and development, DNA damage repair, virus replication and so on, especially suitable for cell proliferation screening experiments of siRNA, miRNA, small molecular compounds and drugs.
Product advantages:
1. Simple: without antigen-antibody reaction, the detection method based on small molecular chemical reaction is simple and efficient, and the reaction only takes a few minutes;
2. Sensitive: without antibodies, the dye is only 1/500 of BrdU antibody, which is easy to spread, and even a single proliferating cell can be accurately detected;
3. Fast: no overnight, eliminating the complex and tedious steps of antigen-antibody reaction, and it takes only five hours to complete the whole detection cycle.
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