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My CartReactivity：Human,Mouse,Rat
Application：IF,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：3845
Host：Rabbit
Qty：
Size：
| Name | Anti-KRAS Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. |
| Target | KRAS |
| Background | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. |
| Swiss Prot | P01116 |
| Gene ID | 3845 |
| Subcellular Location | Cell membrane Cytoplasm Cytoplasmic side Lipid-anchor Cytosol |
| Immunogen | A synthetic peptide of human KRAS |
| Public Immunogen Range | 30-189aa |
| Calculated MW | 22kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.03% Proclin300，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with KRAS antibody diluted at 1:700;Lane: SW620 C6 293T Mouse brain Rat brain.
Immunohistochemistry of paraffin-embedded Human skin cancer with KRAS antibody diluted at 1:100
Immunofluorescence analysis of HeLa using KRAS antibody diluted at 1:100.
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