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My CartReactivity：Human,Rat,Mouse
Application：WB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：968
Host：Rabbit
Qty：
Size：
| Name | Anti-CD68 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. |
| Target | CD68 |
| Background | This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. |
| Swiss Prot | P34810 |
| Gene ID | 968 |
| Subcellular Location | Cell membrane Endosome membrane Lysosome membrane Single-pass type I membrane protein Single-pass type I membrane protein |
| Immunogen | A synthetic peptide of human CD68 |
| Public Immunogen Range | 100-200aa |
| Calculated MW | 31/34/37kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.03% Proclin300，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CD68 antibody diluted at 1:1000;Lane: THP-1 U-937 RAW264.7 Mouse liver Rat liver Rat lung.
Immunohistochemistry of paraffin-embedded Human tonsil with CD68 antibody diluted at 1:100
Immunofluorescence analysis of RAW264.7 cells using CD68 antibody diluted at 1:100.
Immunofluorescence analysis of THP-1 cells using CD68 antibody diluted at 1:100.
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