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CAS:2922-83-0
Appearance:Light yellow crystalline Powder
Storage:2-8℃
Purity:HPLC≥98%
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HPLC-MS
HPLC was performed using an HPLC-MS instrumen for detection and quantitation of tryptophan and kynurenine. The mass scan mode was set to positive and negative ions, and the range was set to 70–1000 m/z. Separation of tryptophan and kynurenine was carried out on an C18 column. Standards and samples were eluted using methyl alcohol and ultrapure water (0.1% formic acid). The injection volume was set to 5 μL, with a solvent flow rate of 0.2 mL/min. The column temperature was set to 40 ℃. The IDA mode was used for data acquisition.
来源文献：Ai Y, Wang B, Xiao S, Luo S, Wang Y. Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan. Int J Mol Sci. 2021 Nov 18;22(22):12428. doi: 10.3390/ijms222212428. PMID: 34830310; PMCID: PMC8623686.
LC-MS/MS
LC-MS/MS conditions Analysis was performed using a liquid chromatographytandem mass spectrometer LC–MS/MS equipped with an ESI source. Separation was performed using a C18 column (250 mm × 4.6 mm × 5 μm). The flow rate was 0.4 ml/min, and the column temperature was maintained at 25℃. The mobile phases were formic acid: water (0.1:100, v/v) as mobile phase A, and methanol:acetonitrile (1:1) and 0.1% formic acid as mobile phase B. The binary gradient elution conditions were: (A:B): 0.01 min–5 min, 70:30→5:95; 5–8 min, 5:95; 8.01 min–16 min, 70:30. Detection was performed using multiple reaction monitoring (MRM) in positive mode, and the optimized MRM parameters for each compound are shown in Table 1. The mass condition parameters were set as: nebulizer gas, 3 L/min; drying gas, 10.0 L/min; heating gas, 10.0 L/min; interface temperature, 30℃; collision-induced dissociation (CID) gas, 230 kPa; DL temperature, 250℃; thermal block temperature, 400℃; interface voltage, ?4.5 kV. The autosampler was kept at 4℃.
来源文献：Xu H, Pan LB, Yu H, Han P, Fu J, Zhang ZW, Hu JC, Yang XY, Keranmu A, Zhang HJ, Bu MM, Jiang JD, Wang Y. Gut microbiota-derived metabolites in inflammatory diseases based on targeted metabolomics. Front Pharmacol. 2022 Sep 27;13:919181. doi: 10.3389/fphar.2022.919181. PMID: 36238574; PMCID: PMC9551995.
HPLC
来源文献：Ai Y, Luo S, Wang B, Xiao S, Wang Y. MiR-126-5p Promotes Tumor Cell Proliferation, Metastasis and Invasion by Targeting TDO2 in Hepatocellular Carcinoma. Molecules. 2022 Jan 10;27(2):443. doi: 10.3390/molecules27020443. PMID: 35056756; PMCID: PMC8779717.
