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My CartReactivity：Rat,Mouse,Human
Application：ICC,IHC,WB,ELISA,FCM,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：7157
Host：Rabbit
Qty：
Size：
| Name | Anti-P53(wt-p53) Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Pig;Cow;Horse;Rabbit;Sheep |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test . ICC 1:100. IF 1:100-500. |
| Target | P53(wt-p53) |
| Background | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. |
| Swiss Prot | P04637 |
| Gene ID | 7157 |
| Subcellular Location | Cytoplasm Endoplasmic reticulum Mitochondrion matrix Nucleus PML body |
| Immunogen | A synthetic peptide of human P53(wt-p53) |
| Public Immunogen Range | 251-310/393aa |
| Calculated MW | 23/30/34/38/44kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with P53 protein(wt-p53) antibody diluted at 1:300;Lane: HepG2 Mcf-7 DU145.
Immunohistochemistry of paraffin-embedded human breast cancer with P53(wt-p53) antibody diluted at 1:400
Immunofluorescence analysis of A549 cells using P53(wt-p53) antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded mouse esophageal with P53 antibody diluted at 1:200.
Immunofluorescence analysis of A549 cell using P53 antibody diluted at 1:100.
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