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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-HSP70 Polyclonal AntibodyReactivity：Rat,Mouse,Human
Application： WB,IHC,ICC,FCM,IF,ELISA
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Host：Rabbit
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| Name | Anti-HSP70 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chicken;Dog;Pig;Rabbit |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100. IF 1:100-500. |
| Target | HSP70 |
| Background | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. |
| Swiss Prot | P0DMV8/P0DMV9 |
| Gene ID | 3303/3304 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human HSP70 |
| Public Immunogen Range | 481-534/641aa |
| Calculated MW | 70kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HSP70 antibody diluted at 1:300;Lane: Mouse Bladder.
Immunofluorescence analysis of A549 cells using HSP70 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cell using HSP70 antibody diluted at 1:100.
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CA9 knockdown enhanced ionizing radiation-induced ferroptosis and radiosensitivity of hypoxic glioma cells
Click to check >>Author：Wenpeng Huang, Yuping He, Shuangyu Yang, Xuefei Xue, Hualong Qin, Ting Sun, Wei Yang
IF：2.6000
Publish_to：INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
PMID：37463506
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