{{cart_num}}
My CartReactivity：Rat,Human
Application：ELISA,FCM,IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1956
Host：Rabbit
Qty：
Size：
| Name | Anti-EGFR Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Mouse;Dog;Pig |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. IF 1:100-500. |
| Target | EGFR |
| Background | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. |
| Swiss Prot | P00533 |
| Gene ID | 1956 |
| Subcellular Location | Nucleus Endosome Plasma membrane Golgi apparatus Endoplasmic reticulum secreted |
| Immunogen | A synthetic peptide of human EGFR |
| Public Immunogen Range | 951-1050/1210aa |
| Calculated MW | 134/45/77/69kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EGFR antibody diluted at 1:1000;Lane: Hela A549 U251 U87MG.
Immunofluorescence analysis of human rectal carcinoma using EGFR antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded human gastric carcinoma with EGFR antibody diluted at 1:200.
Immunofluorescence analysis of human rectal carcinoma using EGFR antibody diluted at 1:200.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
