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My CartReactivity：Rat,Mouse,Human
Application：WB,ELISA,FCM,IF,ICC,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：6657
Host：Rabbit
Qty：
Size：
| Name | Anti-SOX2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chicken;Dog;Pig;Cow;Horse;Rabbit;Sheep;Guinea Pig |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100. IF 1:100-500. |
| Target | SOX2 |
| Background | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. |
| Swiss Prot | P48431 |
| Gene ID | 6657 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human SOX2 |
| Public Immunogen Range | 227-317/317aa |
| Calculated MW | 34kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with SOX2 antibody diluted at 1:300;Lane: Mouse Cerebrum.
Immunohistochemistry of paraffin-embedded Mouse embryo with SOX2 antibody diluted at 1:400
Immunohistochemistry of paraffin-embedded Mouse embryo with SOX2 antibody diluted at 1:400.
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