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My CartReactivity：Human,Rat,Mouse
Application：WB,IF,IHC,FCM,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4744
Host：Rabbit
Qty：
Size：
| Name | Anti-NEFH Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 3μg/Test . IF 1:100-500. |
| Target | NEFH |
| Background | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. |
| Swiss Prot | P12036 |
| Gene ID | 4744 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human NEFH |
| Public Immunogen Range | 1-100/1026aa |
| Calculated MW | 105/112kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NF-H antibody diluted at 1:300;Lane: Mouse Brain Mcf-7.
Immunofluorescence analysis of Rat spinal cord tissue using NEFH antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded mouse cerebellum with NF-H antibody diluted at 1:200.
Immunofluorescence analysis of rat spinal cord tissue using NF-H antibody diluted at 1:200.
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