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My CartReactivity：Human,Rat,Mouse
Application：IF,ICC,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：7080
Host：Rabbit
Qty：
Size：
| Name | Anti-NKX2-1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:100-500. ICC 1:100-500. IF 1:100-500. |
| Target | NKX2-1 |
| Background | Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. |
| Swiss Prot | P43699 |
| Gene ID | 7080 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human NKX2-1 |
| Public Immunogen Range | 201-300/372aa |
| Calculated MW | 42kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TTF1 antibody diluted at 1:1000;Lane: Mouse Thyroid gland Rat Thyroid gland.
Immunohistochemistry of paraffin-embedded Rat brain with NKX2-1 antibody diluted at 1:200
Immunofluorescence analysis of A549 using NKX2-1 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded rat brain tissue with TTF1 antibody diluted at 1:200.
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