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My CartReactivity：Rat,Mouse,Human
Application：IHC,WB,ELISA,FCM,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：13819
Host：Rabbit
Qty：
Size：
| Name | Anti-HIF 2 alpha Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Pig;Cow;Horse |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. IF 1:100-500. |
| Target | HIF 2 alpha |
| Background | Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. |
| Swiss Prot | P97481 |
| Gene ID | 13819 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of mouse HIF 2 alpha |
| Public Immunogen Range | 1-130/874aa |
| Calculated MW | 96kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1米g/Test. IF 1:100-500. |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Mouse brain with HIF 2 alpha antibody diluted at 1:200
Immunofluorescence analysis of Mouse lymph node using HIF 2 alpha antibody at dilution of 1:200.Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded Mouse brain with HIF 2 alpha antibody diluted at 1:500.
Immunofluorescence analysis of mouse lymph node using HIF 2α antibody diluted at 1:200.
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