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My CartReactivity：Mouse,Human
Application：ELISA,FCM,IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：23411
Host：Rabbit
Qty：
Size：
| Name | Anti-SIRT1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Dog;Pig;Horse;Rabbit |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1ug/Test. IF 1:100-500. |
| Target | SIRT1 |
| Background | SirtT1 75 kDa fragment: catalytically inactive 75SirT1 may be involved in regulation of apoptosis. May be involved in protecting chondrocytes from apoptotic death by associating with cytochrome C and interfering with apoptosome assembly. |
| Swiss Prot | Q96EB6 |
| Gene ID | 23411 |
| Subcellular Location | Cytoplasm Mitochondrion Nucleus Nucleus PML body |
| Immunogen | A synthetic peptide of human SIRT1 |
| Public Immunogen Range | 551-650/747aa |
| Calculated MW | 61/82kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with SIRT1 antibody diluted at 1:1000;Lane: Hela NIH/3T3 HepG2 293T Mouse Testis.
Immunohistochemistry of paraffin-embedded Mouse brain with SIRT1 antibody diluted at 1:400
Immunohistochemistry of paraffin-embedded human glioma tissue with SIRT1 antibody diluted at 1:200.
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Exercise reduces hyperlipidemia-induced cardiac damage in apolipoprotein E-deficient mice via its effects against inflammation and oxidative stress
Click to check >>Author：Pei Zuowei, Ji Jun, Gao Yanyan, Wang Heshuang, Wu Yuanyuan, Yang Jin, Yang Qin, Zhang Li
IF：4.6000
Publish_to：Scientific Reports
PMID：37277452
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