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My CartReactivity：Rat,Mouse,Human
Application：IF,IHC,WB,ELISA,FCM,ICC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5599/5601/5602
Host：Rabbit
Qty：
Size：
| Name | Anti-JNK1+JNK2+JNK3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Dog;Pig;Cow |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1ug/Test. ICC 1:100. IF 1:100-500. |
| Target | JNK1+JNK2+JNK3 |
| Background | JNK1(MAPK8) is a member of the MAP kinase family. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7.JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.JNK3 is a neuron-specific form of c-Jun N-terminal kinases. Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis.The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. |
| Swiss Prot | P45983/P45984/P53779 |
| Gene ID | 5599/5601/5602 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | A synthetic peptide of human JNK1+JNK2+JNK3 |
| Public Immunogen Range | 151-250/384aa |
| Calculated MW | 42-47kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF IHC WB ICC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with JNK1+JNK2+JNK3 antibody diluted at 1:300;Lane: Mouse Kidney.
Immunohistochemistry of paraffin-embedded rat kidney with JNK1+JNK2+JNK3 antibody diluted at 1:200.
Immunofluorescence analysis of HeLa cell using JNK1+JNK2+JNK3 antibody diluted at 1:100.
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