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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-TUBB3 Polyclonal AntibodyReactivity：Rat,Mouse,Human
Application：IHC,WB,ELISA,FCM,IF,ICC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：10381
Host：Rabbit
Qty：
Size：
| Name | Anti-TUBB3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Dog;Rabbit |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100. IF 1:200-800. |
| Target | TUBB3 |
| Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. |
| Swiss Prot | Q13509 |
| Gene ID | 10381 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human TUBB3 |
| Public Immunogen Range | 401-450/450aa |
| Calculated MW | 42/50kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TUBB3 antibody diluted at 1:1000;Lane: BV-2.
Immunohistochemistry of paraffin-embedded mouse cerebellum with TUBB3 antibody diluted at 1:200
Immunofluorescence analysis of Mouse brain using TUBB3 antibody diluted at 1:400.
Immunohistochemistry of paraffin-embedded mouse cerebellum with TUBB3 antibody diluted at 1:200.
Immunofluorescence analysis of BV-2 cell using TUBB3 antibody diluted at 1:200.
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