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My CartReactivity：Rat,Mouse,Human
Application：ELISA,FCM,IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3553
Host：Rabbit
Qty：
Size：
| Name | Anti-IL1B Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Dog;Horse;Rabbit |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 2ug/Test. IF 1:100-500. |
| Target | IL1B |
| Background | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
| Swiss Prot | P01584 |
| Gene ID | 3553 |
| Subcellular Location | Cytoplasm Cytoplasmic vesicle Lysosome Secreted autophagosome cytosol exosome |
| Immunogen | A synthetic peptide of human IL1B |
| Public Immunogen Range | 161-269/269aa |
| Calculated MW | 17/31kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with IL-1 Beta antibody diluted at 1:500;Lane: Raw264.7 Mouse Liver.
Immunohistochemistry of paraffin-embedded rat brain tissue with IL-1 Beta antibody diluted at 1:400.
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