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My CartReactivity：Rat,Mouse,Human
Application：ELISA,IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：7033
Host：Rabbit
Qty：
Size：
| Name | Anti-TFF3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Cow |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. IF 1:100-500. |
| Target | TFF3 |
| Background | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. |
| Swiss Prot | Q07654 |
| Gene ID | 7033 |
| Subcellular Location | Cytoplasm Secreted Extracellular matrix |
| Immunogen | A synthetic peptide of human TFF3 |
| Public Immunogen Range | 51-80/94aa |
| Calculated MW | 9kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TFF3 antibody diluted at 1:300;Lane: Mouse Small intestine Rat Small intestine.
Immunohistochemistry of paraffin-embedded Rat colon with TFF3 antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded rat colon with TFF3 antibody diluted at 1:200.
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