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My CartReactivity：Human,Rat,Mouse
Application：ELISA,FCM,IF,ICC,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：25491
Host：Rabbit
Qty：
Size：
| Name | Anti-Nestin Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100. IF 1:100-500. |
| Target | Nestin |
| Background | Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3. |
| Swiss Prot | P21263 |
| Gene ID | 25491 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of rat Nestin |
| Public Immunogen Range | 681-750/1893aa |
| Calculated MW | 109/299kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | ELISA FCM IF ICC IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Rat brain with Nestin antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded rat brain with Nestin antibody diluted at 1:200.
Immunofluorescence analysis of U-251 cell using Nestin antibody diluted at 1:100.
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