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The 3 '→5' exonuclease activity (pfu DNA Polymerase, PrimeSTAR HS DNA Polymerase, etc.) can ensure the fidelity of PCR products. However, the amplified PCR product was A flat terminal DNA fragment without A tail, which could not be directly cloned by TA. Therefore, the flat terminal DNA segment needs to be added A before TA cloning. This kit provides enzymes and buffers required for the addition of flat-terminal DNA fragment A, which can be directly used for the addition of flat-terminal DNA fragment A reaction.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
