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My CartReactivity：Human
Application：IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2657
Host：Rabbit
Qty：
Size：
| Name | Anti-GDF1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:50-200. |
| Target | GDF1 |
| Background | Growth and Differentiation Factor 1 (GDF 1) is a secreted protein in the TGF beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF 1 is synthesized as a preproprotein that produces an approximately 44 kDa proprotein after cleavage of a 29 amino acid (aa) signal peptide. Further proteolysis at a dibasic cleavage site produces a 224 aa propeptide and a secreted 119 aa, 12 15 kDa mature peptide (4). Mature human GDF 1 shares 81% aa sequence identity with mouse and rat GDF 1. GDF 1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF 3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF 1 Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF 1 induced effects (8). GDF 1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF CFC protein such as Cryptic or Cripto (7 9). Loss of function mutations in GDF 1 are associated with a variety of human cardiac development defects (10). |
| Swiss Prot | P27539 |
| Gene ID | 2657 |
| Subcellular Location | Secreted |
| Immunogen | A synthetic peptide of human GDF1 |
| Public Immunogen Range | 90-140/372aa |
| Calculated MW | 39/45kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with GDF1 antibody diluted at 1:100.
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