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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-TUBB3 Polyclonal AntibodyReactivity：Human,Rat,Mouse
Application：WB;IHC;IF,IHC,WB,ELISA,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：10381
Host：Rabbit
Qty：
Size：
| Name | Anti-TUBB3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:8000-12000. IHC 1:25-100. IF 1:50-200. |
| Target | TUBB3 |
| Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. |
| Swiss Prot | Q13509 |
| Gene ID | 10381 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human TUBB3 |
| Public Immunogen Range | 5-55/450aa |
| Calculated MW | 42/50kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:8000-12000. IHC 1:25-100. IF 1:50-200. ELISA 1:8000-80000. |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IF WB;IHC;IF WB IHC ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TUBB3 antibody diluted at 1:10000;Lane: SH-SY5Y,A549,HepG2,PC-3M-IE8,Hela,MCF7,MDA-MB-231,NIH3T3,Human breast cancer,Mouse brain,Mouse testis,Rat brain.
Immunohistochemistry of paraffin-embedded Human breast cancer with TUBB3 antibody diluted at 1:50.
Immunofluorescence analysis of NIH3T3 cells using TUBB3 antibody diluted at 1:100.
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