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My CartReactivity：Rat,Mouse
Application：IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：17345
Host：Rabbit
Qty：
Size：
| Name | Anti-Mki67 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:500-1200. IF 1:500-1200. |
| Target | Mki67 |
| Background | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed. |
| Swiss Prot | E9PVX6 |
| Gene ID | 17345 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of mouse Ki67 |
| Calculated MW | 325/351kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，100 μg/ml BSA and 50% glycerol. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 100ul |
| Reactivity | Rat Mouse |
| Application | IF IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded rat colon with Mki67 antibody diluted at 1:800.
Immunofluorescence analysis of mouse testis using Mki67 antibody diluted at 1:600.
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Lactobacillus brevis alleviates the progress of hepatocellular carcinoma and type 2 diabetes in mice model via interplay of gut microflora,bile acid and NOTCH 1 signaling
Click to check >>Author：Shujia Chen, Ping Han, Qian Zhang, Peiyan Liu, Jie Liu, Lili Zhao, Lianyi Guo, Jia Li
IF：8.7860
Publish_to：Frontiers in Immunology
PMID：37234174
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